IL-10 Monoclonal Antibody (JES3-9D7), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10)
• Applications Reported: The JES3-9D7 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization, and intracellular staining for flow cytometric analysis
• Applications Tested: The JES3-9D7 antibody has been tested for neutralizing IL-10 binding and biological activity
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes
• This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages
• It also enhances B cell survival, proliferation, and antibody production
• Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway
• Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract
• Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis
• Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.