IL-22 Monoclonal Antibody (IL22JOP), Functional Grade, eBioscience™, Invitrogen™

Description

• The MAb IL22JOP reacts with and inhibits the bioactivity of human and mouse IL-22
• IL-22 is a 20 kDa member of the IL-10 cytokine family that is secreted primarily by Th17 cells, NK cells, and other T cells
• Compared to IL-6 or TGF beta, IL-23 can induce greater levels of IL-22 in in vitro-differentiated Th17 cells
• This observation suggests that IL-22 may be secreted by more fully differentiated Th17 cells in vivo
• Recently, it was demonstrated that IL-22 could protect hosts from bacterial infection of the lungs and gut
• Moreover, it has been reported that anti-CD3/CD28-induced production of IL-22 by PBMCs was elevated significantly in asthma patients compared to control patients
• Flow cytometric analysis also showed that the frequencies of IL-17+IL-22+ CD4 T cells were increased in PBMCs from patients with ankylosing spondylitis and rheumatoid arthritis
• IL22JOP is published to recognize rhesus IL-22
• IL-22 also known as IL-10-related T-cell derived inducible factor, is an alpha helical cytokine and is considered a member of the IFN-IL-10 family, which includes IL-19, IL-20, IL-24, IL-26, IL-28, IL-29, and the type I and II interferons
• IL-22 is produced mainly by activated T cells and NK cells
• In humans, the IL-22 gene is located on the q arm of chromosome 12, and is structurally related to IL10
• IL-22 acts by engaging the heterodimeric receptor complex consisting of primary receptor IL-22R1 and accessory receptor IL-10R2
• IL-22R1 also binds IL-20 and IL-24; IL-10R2 also binds IL-10, IL-27, IL-28, and IL-29
• Binding of IL-22 to its receptor complex induces signal transduction, particularly via the JAK-STAT pathway
• In addition to the membrane-bound IL-22R1/IL-10R2 complex, a soluble single-chain IL-22 receptor termed IL-22BP has been found to antagonize IL-22 binding and signaling
• IL-22 appears not to directly influence immune cells, and major targets of the cytokine appear to be nonimmune cells, such as cells of the skin, digestive and respiratory system, as well as hepatocytes, and keratinocytes
• IL-22 has been described as an effector cytokine of the Th17 lineage
• Along with IL-17A and IL-17F, IL-22 regulates genes associated with innate immunity of the skin
• IL-17A, IL-17F and IL-22 are all co-expressed by Th17 cells, however, they are differentially regulated
• The effects of IL-22 include induction of acute phase reactants and antimicrobial proteins, as well as increasing the mobility of keratinocytes
• IL-22 is highly expressed during chronic inflammation, and found to activate intracellular kinases and transcription factors
• IL-22 is critical for host defense against infections of extracellular pathogens, and promotes wound-healing responses
• IL-22 is upregulated in activated T cells
• IL-22 has been reported to mediate IL-23-induced acanthosis and dermal inflammation through activation of STAT3.