TIGIT Monoclonal Antibody (MBSA43), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The MBSA43 monoclonal antibody reacts with human T cell immunoreceptor with Ig and ITIM domains (TIGIT), which is also known as VSTM3 or WUCAM
• This 26 kDa protein is a member of the poliovirus receptor (PVR) family
• The expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but it is not expressed on B cells or naive CD4+ T cells
• TIGIT may be upregulated on naive CD4+ T cells upon activation
• TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK and T cells with antigen presenting cells, fibroblasts, and endothelial cells that express PVR and PVR-like proteins
• MBSA43 is reported to crossreact with Rhesus macaque
• Applications Reported: This MBSA43 antibody has been reported for use in flow cytometric analysis
• In a functional assay, MBSA43 enhances IFN gamma secretion following TCR activation
• Applications Tested: This MBSA43 antibody has been tested by flow cytometric analysis
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Endotoxin: Less than 0
• Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.