CD278 (ICOS) Monoclonal Antibody (7E.17G9), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family
• A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response
• ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases
• ICOS may be involved in the development of Th2 cells
• Applications Reported: 7E.17G9 has been reported for use in flow cytometric analysis and for inhibition of ligand binding in functional assays
• Applications Tested: The 7E.17G9 antibody has been tested by flow cytometric analysis of unstimulated and Con A activated (3 day) mouse splenocytes and can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family
• It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation
• ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.