CD7 Monoclonal Antibody (4H9), APC, eBioscience™, Invitrogen™

Description

• Description: This 4H9 monoclonal antibody reacts with human CD7 (also known as Leu-9 or gp40), a 40-kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily
• This molecule is expressed on peripheral blood T lymphocytes and natural killer cells, as well as on lymphoid and myeloid lineage precursors during adult hematopoiesis
• Capable of providing a costimulatory signal and associating with CD3 and CD45, CD7 is involved in T cell activation
• CD7 has also been detected on numerous T cell-derived leukemias and lymphomias
• In fact, because CD7 expression is greater on CD4+ T cells in classical Hodgkin's lymphoma than in benign reactive lymph adenopathy, this molecule may be used as a diagnostic for Hodgkin's lymphoma
• Blocking assays demonstrate that this clone recognizes the same epitope as clone 124-1D1
• Applications Reported: This 4H9 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 4H9 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily
• CD7 contains N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxy-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects
• CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia
• Further, CD7 is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells
• Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation
• CD7 associates directly with phosphoinositol 3'-kinase
• CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation
• Expression of CD7 is an important marker used in leukemia diagnostics.