CD49c (Integrin alpha 3) Monoclonal Antibody (P1B5), APC, eBioscience™, Invitrogen™

Description

• Description: This P1B5 monoclonal antibody reacts with human CD49c (also known as Integrin alpha 3)
• Integrins constitute a family of cell surface receptors that mediate adhesion between cells or between a cell and the extracellular matrix
• These proteins are heterodimers consisting of two distinct subunits, alpha and beta
• Integrins are expressed on hematopoietic, non-hematopoietic, and cancer cells
• Integrin-mediated adhesion is regulated by changes in ligand affinity (or activation), cell shape, and integrin clustering and diffusion
• Integrin alpha 3 forms a complex with the common beta 1 subunit to form the alpha3beta1 integrin
• Studies have demonstrated the involvement of this integrin in wound healing and epithelial migration
• The P1B5 monoclonal antibody has been reported to affect adhesion to type I and VI collagens, as well as fibronectin and laminin
• Applications Reported: This P1B5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This P1B5 antibody has been pre-titrated and tested by flow cytometric analysis of HeLa cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• ITGA3 (INTEGRIN, ALPHA-3), also called CD49C, VLA3 or GAPB3, is a protein that in humans is encoded by the ITGA3 gene
• ITGA3 is an integrin alpha subunit which is also a member of the family of cell surface adhesion molecules
• ITGA3 is mapped to chromosome 17 and its exact cytogenetic location is 17q21.33
• ITGA3 makes up half of the alpha3beta1 integrin duplex that plays a role in neural migration and corticogenesis together with beta-1 subunit
• A functional link between DAB1 phosphorylation and ITGA3 signaling drives the timely detachment of migrating neurons from radial glial fibers
• Expression of human ITGA3 increased the infectivity of virus for Chinese hamster ovary cells
• ITGA3 also contains 13 potential N-glycosylation sites, 2 potential cleavage sites, and the 7 N-terminal repeating units characteristic of ITGAs
• Recombinant ITGA3 is expressed as a 150-kD protein as the same size as the native protein by the western blot analysis.