CD58 (LFA-3) Monoclonal Antibody (TS2/9), APC, eBioscience™, Invitrogen™

Description

• Description: The TS2/9 antibody recognizes human CD58, which is also known as Lymphocyte Associated Antigen-3 (LFA-3)
• CD58 exists both as a transmembrane protein as well as a GPI-linked extracellular protein
• It has been shown that cells can express both forms concurrently
• In addition, soluble CD58 has also been observed in serum, urine and synoval fluid of rheumatoid arthritis patients
• CD58 is found on non-hematopoietic and most hematopoietic cells
• Binding to its ligand CD2, which is present on T cells, initiates a signaling pathway which includes phosphorylation and results in release of cytokines
• CD58 has been shown to be important in adhesion to various cell types, T cell activation, cytokine production and cytoxicity
• The TS2/9 antibody has been reported to induce signaling events upon crosslinking
• The epitope is in domain 1 of the immunoglobulin region
• Applications Reported: This TS2/9 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This TS2/9 antibody has been pre-titrated and tested by flow cytometric analysis of normal peripheral blood cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• This gene encodes a member of the immunoglobulin superfamily
• The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes
• The protein is localized to the plasma membrane
• Alternatively spliced transcript variants have been described
• [provided by RefSeq, Jan 2009]