CD151 Monoclonal Antibody (50-6), APC, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 50-6 recognizes human CD151 also known as PETA-3 (platelet-endothelial cell tetraspan antigen-3)
• CD151 has a molecular weight of 29 kDa and like other tetraspanin family members, CD151 contains four hydrophobic transmembrane domains, two short cytoplasmic tails, and one small and one large extracellular loop
• CD151 is expressed on platelets, megakaryocytes, endothelial cells, at the cell-cell junctions, as well as epithelial cells where it localizes to basolateral surfaces exposed to extracellular matrix
• The tetraspanins play a role in cell adhesion and migration; CD151 interacts with integrinalpha3 beta1 and integrinalpha6 beta4
• In particular CD151 forms a functional complex with c-Met and integrin alpha3 and alpha6 in human salivary gland cancer cells
• Additionally it can interact with CD46
• Together these interactions are thought to play a role during early metastasis as a potential positive effector
• The 50-6 monoclonal antibody inhibits in vivo metastasis of a human epidermal carcinoma cell line, HEp-3
• Applications Reported: This 50-6 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 50-6 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family
• Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains
• The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility
• This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins
• It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function
• This protein enhances cell motility, invasion and metastasis of cancer cells
• Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene.