CD229 (Ly-9) Monoclonal Antibody (HLy9.25), APC, eBioscience™, Invitrogen™

Description

• Description: This HLy9.25 monoclonal antibody reacts with human CD229 (also known as Ly9 or SLAMF3), an approximately 120-kDa member of the Signaling Lymphocyte Activation Molecule (SLAM) immunoglobulin superfamily
• This type I transmembrane glycoprotein is expressed on a variety of immune cell types, including thymocytes, T and B lymphocytes, and natural killer cells
• Expression of CD229 is also detected on T and B cell malignancies, including Jurkat and Burkitt lymphoma cells, respectively
• CD229 has been shown to interact with SH2-binding phosphatases and the adaptor proteins SLAM-associated protein (SAP) and EAT-2
• Moreover, CD229 localizes to the immunological synapse following T cell activation
• Applications Reported: This HLy9.25 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HLy9.25 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells
• This can be used at 5 μL (0.03 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g
• CD48 and CD224
• Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells
• High levels of CD229 are found on T and B cells, where its expression increases during their maturation
• It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes
• CD229 has been also reported on mouse monocytes and NK cells
• CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.