CD272 (BTLA) Monoclonal Antibody (8F4), APC, eBioscience™, Invitrogen™

Description

• Description: The BTLA antibody reacts with mouse BTLA, B and T lymphocyte attenuator from both BALB/c and C57Bl/6 strains
• BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in thymus and mature, but not immature bone marrow-derived dendritic cells
• BTLA is implicated as a negative regulator of the activation and/or function of various hemopoietic cell types
• It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction
• Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4+ and CD8+ single-positive (SP) thymocytes (Hurchla et al)
• However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes
• It is not understood why there is a discrepancy in thymocyte staining
• However, in staining of splenocytes and bone marrow cells, clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7
• Applications Reported: This 8F4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 8F4 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• BTLA or B and T-lymphocyte attenuator is a member of the co-inhibitory receptors of the CD28 superfamily
• BTLA is a lymphocyte inhibitory receptor which inhibits lymphocytes during immunes responses
• BTLA is constitutively expressed on most CD4+ and CD8+ T cells and its expression progressively decreases upon T cell activation
• It remains expressed on Th1 cells, but not Th2 cells
• BTLA is a unique co-receptor that interacts with the tumor necrosis factor receptor superfamily member herpesvirus entry mediator (HVEM)
• This interation is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response.