GARP Monoclonal Antibody (YGIC86), APC, eBioscience™, Invitrogen™

Description

• Description: The YGIC86 monoclonal antibody reacts with mouse Glycoprotein A Repetitions Predomit (GARP, also known as Lrrc32 or Garpin)
• GARP is an approximately 80 kDa glycoprotein that is expressed on the cell surface
• Using northern blot, RT-PCR or microarray analyses, the expression of GARP has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas
• Protein expression has been observed on megakaryocytes, platelets and activated regulatory T (Treg) cells
• The expression of GARP on Treg cells has been reported to be necessary for the suppressive function of Treg cells, possibly related to its role as a cell surface receptor for LAP/TGF beta
• When looking for expression of GARP on platelets, it is recommended to use the Foxp3 Staining Buffer Set (cat
• 00-5523) to fix the platelets before staining with this YGIC86 monoclonal antibody
• Fixation/permeabilization is not necessary when staining Treg cells
• YGIC86 does not cross-react to rat GARP
• Applications Reported: This YGIC86 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This YGIC86 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes
• This can be used at less than or equal to 0.125 µg per test
• A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region
• GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation
• Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.