GARP Mouse anti-Human, Brilliant Violet 605, Clone: 7B11 (also known as CMSSC-7B11), BD Optibuild™

Description

• The 7B11 (also known as CMSSC-7B11) monoclonal antibody specifically binds to human GARP (Glycoprotein A repetitions predominant)
• The LRRC32 /(Leucine rich repeat containing 32) gene encodes the 662 amino acid-residue, 80 kDa transmembrane GARP glycoprotein that has an extracellular region composed primarily of 20 leucine-rich repeats
• GARP is specifically expressed on Treg cells activated through the T cell receptor (TCR)
• Ectopic expression of GARP in human naοve T cells inhibited their proliferation and cytokine secretion upon TCR activation
• Remarkably, GARP over-expression in naοve T cells induced expression of FoxP3 and endowed them with a partial suppressive function
• The extracellular, but not the cytoplasmic region, of GARP was necessary for these functions
• GARP serves as a receptor for latent TGF-beta which may play a role in the suppressive action of Treg cells
• GARP is also expressed on platelets and other tissues, however the function on these cells is not known
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.