EGF Receptor Mouse anti-Human, Brilliant Violet 650, Clone: EGFR.1, BD Optibuild™

Description

• The EGFR.1 monoclonal antibody specifically binds to the epidermal growth factor receptor (EGF-R)
• The EGF-R is a transmembrane glycoprotein of approximately 170 kDa that is expressed on most cells
• It consists of a glycosylated extracellular domain which binds to epidermal growth factor (EGF), a transmembrane domain, and an intracellular domain with tyrosine-kinase activity essential for signal transduction
• The EGF-R plays an important role in the growth and differentiation of many cellular types
• Transforming growth factorα(TGFα), vaccinia virus growth factor, and related growth factors can also bind to and signal through the EGF-R
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.