H-2Dd Mouse anti-Mouse, Brilliant Violet 421, Clone: 34-2-12, BD Optibuild™

Description

• The 34-2-12 antibody (also known as 34-2-12S) recognizes theα3 domain of the H-2D[d]
• The binding of the antibody to its epitope is independent of theα1 andα2 domains andβ2 microglobulin
• It cross-reacts with cells of the C3H.LG/Ckc strain
• Reactivity with other haplotypes (eg, b, f, k, p, q, r, s /) has not been observed
• Soluble mAb 34-2-12 blocks binding of the Ly-49A-expressing T lymphoma EL4 to immobilized H-2D[d]
• However, further studies utilizing this mAb indicate that theα3 domain is not involved in the interaction between Ly-49A, or Ly-49G2, and H-2D[d]
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.