Lambda light chain Monoclonal Antibody (1-155-2), APC, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 1-155-2 recognizes the lambda light chain of human immunoglobulin
• Approximately 22-kDa, the Ig lambda chain is comprised of one variable and one constant region
• Two lambda chains together with the heavy chain comprise the whole Ig structure
• Expression of Ig lambda is typically found in less than half of B lymphocytes, and has been associated with neoplastic lymphomas
• Applications Reported: This 1-155-2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 1-155-2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa)
• Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region
• The ratio of kappa to lambda is 70:30
• Antibodies to the kappa light chain are reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas
• Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.