CD28 Monoclonal Antibody (37.51), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells
• CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells
• Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells
• Applications Reported: This 37.51 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 1 µg per test
• A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Light sensitivity: This tandem dye is sensitive photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
• Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein
• CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes
• Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells
• In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death
• In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28
• In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR
• The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells
• Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary’s Disease.