USP7 Polyclonal Antibody, Bethyl Laboratories

Description

• The recommended shelf life for this product is 1 year from date of receipt
• Application Note: For IHC, many tissues will not require epitope retrieval
• However to boost weak staining epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
• Based on 100% sequence identity, this antibody is predicted to react with Rat USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates
• Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins
• HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53
• Normally, p53 levels are kept low due to Mdm2-mediated ubiquitylation and degradation of p53
• Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress
• Another important role of HAUSP function involves the oncogenic stabilization of p53
• Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process
• An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.