Nucleolin/NCL Polyclonal Antibody, Bethyl Laboratories

Description

• The recommended shelf life for this product is 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
• Based on 100% sequence identity, this antibody is predict Nucleolin, which is identical to human DNA helicase IV, is a major nucleolar phosphoprotein which is associated with preribosomal RNA and is implicated in the early stage of preribosomal RNP assembly and processing
• This 100 kDa protein has three major domains: a N-terminal domain comprised of long acidic stretches interspersed with basic repeats, similar to the structure of a high mobility group-type protein, a central domain that contains four RNA binding elements, a C-terminal domain approximately 85 amino acids long that is rich in glycine, arginine, and phenylalanine residues
• Nucleolin fluctuates in parallel to DNA synthesis; intact 100 kDa protein is the major species in actively dividing cells, whereas the degraded forms are relativley abundant in nondividing cells
• Nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes
• Nucleolin also interacts directly with DNA topoisomerase I
• It is located mainly in dense fibrillar regions of the nucleolus
• Nucleolin is the major nucleolar protein of growing eukaryotic cells
• It is found associated with intranucleolar chromatin and preribosomal particles
• It induces chromatin decondensation by binding to histone H1
• It is thought to play a role in pre-rRNA transcription and ribosome assembly
• It interacts with APTX and contains 4 RNA recognition motif (RRM) domains.