Topo II Beta Polyclonal Antibody, Bethyl Laboratories

Description

• The recommended shelf life for this product is 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
• This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription
• This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication
• It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA
• Two forms of this enzyme exist as likely products of a gene duplication event
• The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17
• The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance
• Reduced activity of this enzyme may also play a role in ataxia-telangiectasia
• Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described.