UNG Rabbit anti-Human, Polyclonal, Bethyl Laboratories

Description

• The recommended shelf life for this product is 1 year from date of receipt
• The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase
• These forms are generated by alternative splicing and the use of two differentially regulated promoters, PA and PB (Slupphaug et al., 1993; Nilsen et al., 1997) The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively) and as a result the corresponding mRNAs are not resolved as two species in gel electrophoresis (Huag et al., 1998)
• Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence that is not necessary for catalytic activity
• A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination
• For example, UNG2 removes misincorporated dUMP residues
• The level and expression pattern of UNG1 and UNG2 differs between cell and tissue type (Huag et al
• 1998)
• Additionally, the expression of UNG is cell cycle regulated (Nagelhus et al
• 1995).The expression and activity of UNG has been found in general to be higher in proliferating as compared to nonproliferating tissues and cells (reviewed in Kruman et al
• 2004)
• UNG1 is a 304 amino acid protein
• UNG2 is a 313 amino acid protein.