CD45 Polyclonal Antibody, Bethyl Laboratories

Description

• The recommended shelf life for this product is 1 year from date of receipt
• Application Note: For ICC, acetone or Formaldehyde fixation is recommended
• Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells
• For IHC, epitope retri CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
• CD45 is absent on non-hematopoietic cell lines, normal and maligt, non-hematopoietic tissues
• CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
• CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains
• CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells
• CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
• CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling
• CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.