Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), PE-Cyanine5.5, eBioscience™, Invitrogen™

Description

• Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1
• A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow
• While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations
• eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4)
• It is recommended that 1A8-Ly6G (cat
• 9668) be used when looking at Ly-6G specific targets
• Applications Reported: This RB6-8C5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow and splenocyte suspensions
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm
• They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments
• In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes
• Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.