INA Rabbit anti-Human, Polyclonal, Bethyl Laboratories

Description

• The recommended shelf life for this product is 1 year from date of receipt
• Alpha internexin is a Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits
• On SDS-PAGE gels it runs with an apparent molecular weight of 64 to 66 kDa, with some species variability, although the real molecular weight is about 55kDa; as with the other neurofilament subunits the presence of highly negatively charged sequences results in reduction of SDS-PAGE mobility
• a-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization
• It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds
• Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H
• In some mature neurons a-internexin is the only neurofilament subunit expressed
• Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture
• In addition, the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cells committed to the neuronal lineage
• In addition, recent studies show a marked up-regulation of a-internexin during neuronal regeneration.