CD15 Monoclonal Antibody (HI98), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X
• This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes
• Monocytes express this structure at varying degree while lymphocytes are negative
• Applications Reported: This HI98 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HI98 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• Please make sure that your instrument is capable of detecting this fluorochrome
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g
• granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells
• There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x
• Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction
• This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells
• Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins
• CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.