CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1)
• CD90 is a 25-35 kDa receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells
• CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+
• The CD90+CD34+ population is enriched for cells capable of long-term culture
• CD90 is involved in regulation of adhesion and signal transduction by T cells
• Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBio5E10 (5E10) antibody has been pre-titrated and tested by flow cytometric analysis of the HEL cell line
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• Please make sure that your instrument is capable of detecting this fluorochrome
• CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family
• CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts
• CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain
• CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons
• Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor
• Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.