TCR DO11.10 Monoclonal Antibody (KJ1-26 (KJ126, KJI-26, KJI26)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested.The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-A^d major histocompatibility molecules.The use of DO11.10 and KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity
• Applications Reported: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been tested by flow cytometric analysis of DO.11 cells
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm)
• Please make sure that your instrument is capable of detecting this fluorochrome
• For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative
• TCR DO11 (DO11.10) is a receptor found on lymphocytes of DO11.10 transgenic mice and T-cell receptors of BALB/c-derived DO11.10 and DO11.10.24 T cell hybridomas
• The DO11.10 TCR recognizes a C-terminal ovalbumin peptide (OVA 323-339) in the context of I-Ad major histocompatibility molecules
• The use of antibodies against DO11.10 have proven to be instrumental in describing many of the intricate details of T cell immunity, including T cell activation, development, and function.