CD83 Monoclonal Antibody (Michel-17 (Michel17)), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The Michel-17 monoclonal antibody reacts with mouse CD83, a 45kDa cell surface glycoprotein and a member of the Ig superfamily
• The mouse CD83 antigen is expressed predominantly on mature DC and activated lymphocytes
• Cross-linking of CD83 with Michel-17 on DC or activated T cells does not induce any activation signal
• CD83 plays an important role in T cell development through interaction with its ligand
• CD83-Ig protein has revealed the presence of a CD83 ligand expressed mainly by B220+ cells in mouse spleen
• Applications Reported: This Michel-17 (Michel17) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This Michel-17 (Michel17) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD83 cell surface antigen is a 40-45kD glycoprotein expressed by peripheral blood dendritic cells
• Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA
• In immunohistology, CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres
• CD83 is also expressed by Langerhan's cells in the skin
• The CD83 antigen is a 186-amino-acid single-chain glycoprotein and this molecule is a member of the immunoglobulin superfamily that is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail
• CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein
• However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage
• CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages
• They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules
• Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.