CD25 Monoclonal Antibody (BC96), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha)
• CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes
• By itself, CD25 binds IL-2 only with low affinity
• However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor
• CD25 plays a role in lymphocyte differentiating and activation/proliferation
• Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of human normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• When staining for CD25 (with emission at 518nm) it is recommended to use Alexa Fluor 488 conjugated antibodies, rather than FITC for optimal detection
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes
• The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7)
• IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor
• Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor
• The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene
• IL2 is primarily produced by mature T cells
• IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death
• IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation
• The targeted disruption of a similar gene in mice leads to μgcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli
• Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.