CD144 (VE-cadherin) Monoclonal Antibody (16B1), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5
• The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions
• VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues
• It is thought to play a role in vascular permeability and remodeling
• Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 16B1 antibody has been pre-titrated and tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC)
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer
• VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail
• Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions
• An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.