MAP1LC3A Monoclonal Antibody (OTI2D5), TrueMAB™, OriGene

Description

• Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation
• During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment
• There are 16 proteins participating in the autophagy pathway in human
• The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3
• It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development
• LC3 is the only known mammalian protein identified that stably associate with the autophagosome membranes
• LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction
• The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.