MDM2 Monoclonal Antibody (OTI25B9), TrueMAB™, OriGene

Description

• MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53
• MDM2 is located on chromosome 12 on the q arm
• Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53
• Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53
• MDM2 has also been shown to negatively regulate p53 function
• MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function
• Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity
• Further, MDM2 plays important roles in apoptosis and cell cycle
• MDM2 is over expressed in a wide range of human maligcies including soft tissue carcinomas and breast cancer
• In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5
• More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.