CD26 Monoclonal Antibody (M-A261), FITC, eBioscience™, Invitrogen™

Description

• Description: CD26 is a 110 kD type II membrane protein encoded by the DPP4 gene
• It is an antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis
• It also plays an integral role as a costimulatory molecule in T cell activation
• CD26 may interact with extracellular matrix proteins such as fibronectin or collagen, CD45 and ADA
• Immunohistochemistry: The antibody can be used to stain cryostat sections
• Recommended for BMS143 is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP), or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections
• Flow cytometry: BMS143 is also suitable as primary antibody in staining for FACS analysis, BMS143FI can be used for direct staining of cells, BMS143BT can be used in a two-step procedure using the biotin-(strept-)avidin system
• Recommended dilution is 1:20 as determined on A704 cells
• Immunoprecipitation: BMS143 is also suitable for immunoprecipitation
• Optimal dilutions should be determined by the individual laboratory for each application
• Applications Tested: Flow Cytometry
• Purity: >95%
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser CD26 (dipeptidyl peptidase IV, DPP IV), adenosine deaminase (ADA) binding protein) is a homodimeric atypical serine protease belonging to the prolyl oligopeptidase family
• CD26 is expressed on lymphocyte cells and is upregulated during T-cell activation
• CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia
• CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection
• CD26 identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26
• Further, CD26 is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides
• Alterations in CD26 peptidase activity are characteristic of malignant transformation, and the enzymatic activity increases dramatically with tumor grade and severity
• CD26 is expressed in various blood cell types, but also in cells that are histogenetically related to activated fibroblasts
• Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.