50-194-3794
DYKDDDDK Monoclonal Antibody (29E4.G7), Rockland™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-194-3794-Each-of-1 | In Stock | ₹ 55,932.28 |
50-194-3794 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 55,932.28
GST (18%): ₹ 10,067.81
Total Price: ₹ 66,000.09
Antigen
DYKDDDDK
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species
Mouse
Quantity
500 μg
Primary or Secondary
Primary
Product Type
Antibody
Isotype
IgG2a κ
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone
29E4.G7
Conjugate
Unconjugated
Gene Alias
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status
RUO
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Form
Liquid
Target Molecule
Tag
Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- The purified antibody is directed against the FLAGTM motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins
- This monoclonal anti-FLAGTM tag antibody detects over-expressed proteins containing the FLAGTM epitope tag
- In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins
- DYKDDDDK Tag (FLAG™ tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology
- The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended
- A FLAG tag can be used in many different assays that require recognition by an antibody
- If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence
- Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis
- Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein
- The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function
- The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins
- FLAG is a trademark of Sigma-Aldrich Biotechnology LP
Compare Similar Items
Show Difference
Antigen: DYKDDDDK
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 500 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG2a κ
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: 29E4.G7
Conjugate: Unconjugated
Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: Tag
Antigen:
DYKDDDDK
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
500 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG2a κ
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
29E4.G7
Conjugate:
Unconjugated
Gene Alias:
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen:
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
Tag
Antigen: Ovalbumin
Classification: Polyclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Rabbit
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: __
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: __
Conjugate: Unconjugated
Gene Alias: Allergen Gal d II; clade (ovalbumin); Egg albumin; Gal d 2; member 14; OVAL; Ovalbumin; ovalbumin (SERPINB14); Plakalbumin; serpin peptidase inhibitor; SERPINB14
Immunogen: Ovalbumin [Hen Egg White]
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: __
Antigen:
Ovalbumin
Classification:
Polyclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Rabbit
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
__
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
__
Conjugate:
Unconjugated
Gene Alias:
Allergen Gal d II; clade (ovalbumin); Egg albumin; Gal d 2; member 14; OVAL; Ovalbumin; ovalbumin (SERPINB14); Plakalbumin; serpin peptidase inhibitor; SERPINB14
Immunogen:
Ovalbumin [Hen Egg White]
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
__
Antigen: Lysozyme
Classification: Polyclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Rabbit
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: __
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: __
Conjugate: Unconjugated
Gene Alias: 1,4-beta-N-acetylmuramidase C; 1700038F02Rik; AI326280; bA534G20.1; c-type lysozyme; KAAG648; LYC2; Lys; Lysm; lysozyme; lysozyme (renal amyloidosis); lysozyme 1; lysozyme 2; Lysozyme C; Lysozyme C type M; Lysozyme C type P; Lysozyme C-1; Lysozyme C-2; lysozyme C-3; lysozyme d1; lysozyme F1; lysozyme like 1; lysozyme-like 1; Lysozyme-like protein 1; lysozyme-like protein 2; Lysz; Lyz; Lyz1; Lyz2; LYZD1; LYZF1; Lyzl1; Lyzs; LZM; Lzm-s1; Lzp; Lzp-s; P lysozyme structural; PRO1278; renal amyloidosis; RGD1559869; UNQ648/PRO1278
Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: __
Antigen:
Lysozyme
Classification:
Polyclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Rabbit
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
__
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
__
Conjugate:
Unconjugated
Gene Alias:
1,4-beta-N-acetylmuramidase C; 1700038F02Rik; AI326280; bA534G20.1; c-type lysozyme; KAAG648; LYC2; Lys; Lysm; lysozyme; lysozyme (renal amyloidosis); lysozyme 1; lysozyme 2; Lysozyme C; Lysozyme C type M; Lysozyme C type P; Lysozyme C-1; Lysozyme C-2; lysozyme C-3; lysozyme d1; lysozyme F1; lysozyme like 1; lysozyme-like 1; Lysozyme-like protein 1; lysozyme-like protein 2; Lysz; Lyz; Lyz1; Lyz2; LYZD1; LYZF1; Lyzl1; Lyzs; LZM; Lzm-s1; Lzp; Lzp-s; P lysozyme structural; PRO1278; renal amyloidosis; RGD1559869; UNQ648/PRO1278
Immunogen:
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
__
Antigen: Ferritin
Classification: Polyclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Rabbit
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: __
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: __
Conjugate: Unconjugated
Gene Alias: apoferritin; Cell proliferation-inducing gene 15 protein; Ferritin H subunit; Ferritin heavy chain; ferritin heavy chain 1; Ferritin heavy chain, N-terminally processed; Ferritin L subunit; ferritin L-chain; Ferritin light chain; ferritin light polypeptide-like 3; Ferritin subunit H; ferritin, heavy polypeptide 1; ferritin, light polypeptide; FHC; FTH; Fth1; FTHL6; FTL; HFE5; H-ferritin; HFt; L apoferritin; LFTD; MFH; MGC71996; NBIA3; OK/SW-cl.84; PIG15; placenta immunoregulatory factor; PLIF; proliferation-inducing protein 15
Immunogen: Ferritin [Human Spleen]
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: __
Antigen:
Ferritin
Classification:
Polyclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Rabbit
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
__
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
__
Conjugate:
Unconjugated
Gene Alias:
apoferritin; Cell proliferation-inducing gene 15 protein; Ferritin H subunit; Ferritin heavy chain; ferritin heavy chain 1; Ferritin heavy chain, N-terminally processed; Ferritin L subunit; ferritin L-chain; Ferritin light chain; ferritin light polypeptide-like 3; Ferritin subunit H; ferritin, heavy polypeptide 1; ferritin, light polypeptide; FHC; FTH; Fth1; FTHL6; FTL; HFE5; H-ferritin; HFt; L apoferritin; LFTD; MFH; MGC71996; NBIA3; OK/SW-cl.84; PIG15; placenta immunoregulatory factor; PLIF; proliferation-inducing protein 15
Immunogen:
Ferritin [Human Spleen]
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
__