Collagen Type V Polyclonal Antibody, Rockland™

Description

• Store vial at 4°C prior to opening
• This product is stable at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards
• Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues
• This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV or VI collagens
• Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible
• Type I collagen, the fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage
• Mutations in this gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, and idiopathic osteoporosis
• Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor
• Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.