HAUSPRabbit anti-Human, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Directed against human HAUSP protein
• A BLAST analysis was used to suggest cross-reactivity with HAUSP protein from human, rat, mouse, chimpanzee, bovine and dog based on 100% homology with the immunizing sequence
• Expect partial reactivity with HAUSP from chicken (92%, 11/12) and zebrafish (83%, 10/12) sources based on partial sequence homology
• Reactivity against homologues from other sources is not known
• USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates
• Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins
• HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53
• Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53
• Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress
• Another important role of HAUSP function involves the oncogenic stabilization of p53
• Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process
• An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.