CDC2 p34Rabbit anti-Human, Mouse, Rat, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Antiserum will specifically react with a 34 kDa cdc2 protein from human, rat and mouse tissue
• No reaction was observed against other related cyclin dependent kinases
• Cross reactivity with cdc2 from other species may also occur
• CDK1 (CDC2, P34CDC2) is a member of the Ser/Thr protein kinase family
• CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle
• Mitotic cyclins stably associate with this protein and function as regulatory subunits
• The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle
• The phosphorylation and dephosphorylation of CDK1 also play important regulatory roles in cell cycle control
• Destruction of cyclin B during metaphase results in inactivation of CDK1, allowing mitotic exit and cell division
• CDK1 also contributes to the control of DNA replication and can be inhibited by several transcriptional targets of p53, such as p21WAF
• The ability of CDK1 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2
• Research studies have shown that expression of CDK1 in the brain has been linked to both neurogenesis and apoptosis
• In vertebrates, multiple cyclins like CDK1 have been identified, including a total of eight such regulatory proteins in mammals
• Alternatively spliced transcript variants encoding different isoforms of CDK1 have been found.