MMTV Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• A BLAST analysis was used to suggest cross-reactivity with MMTV from mouse based on a 100% homology with the immunizing sequence
• Reactivity against homologues from other sources is not known
• Mouse mammary tumor virus capsid is a polyprotein that is cleaved by aspartyl protease during or after the release of the virion from the plasma membrane
• After entering the cell, reverse transcriptase converts the viral dimeric RNA genome into dsDNA in the cytoplasm
• Displaying DNA polymerase activity and RNase H activity, this enzyme copies either DNA or RNA templates and cleaves the RNA strand of the RNA-DNA heteroduplex in a partially processive 3' to 5' endonucleasic mode
• tRNS binds to the primer at the 5' end of the viral RNA
• Reverse transcriptase uses the 3' end of the tRNA primer to perform a short round of RNA-dependent minus-strand DNA synthesis proceeding through the U5 region and ending after the repeated region
• This RNA-DNA heteroduplex is digested by the RNase H and hybridizes with the identical R region at the 3' end of the viral RNA
• RNase H then digests the RNA template except for a polypurine tract situated at the 5' end of the genome
• RNase H probably can proceed both in a polymerase-dependent and a polymerase-independent mode
• Reverse transcriptase also performs DNA-directed plus-strand DNA synthesis using the polypurine tract that has not been removed by RNase H as primers
• Polypurine tract and tRNA primers are then removed by RNase H and the 3' and 5' ssDNA primer binding site regions hybridize to form a circular dsDNA intermediate
• Strand displacement synthesis by reverse transcriptase to the primer binding site and polypurine tract ends produces a blunt ended, linear dsDNA copy of the viral genome that includes long terminal repeats at both ends
• Anti-MMTV Antibody is ideal for researchers interested in Cancer, Enzyme, and Viral research.