ArginaseRabbit anti-Bovine, Polyclonal, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Arginase [Bovine Liver]
• Cross reactivity against Arginase from other tissues and species may occur but have not been specifically determined
• Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle
• The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation
• By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production
• In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells
• In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported
• In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a)
• Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas
• Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.