Plasminogen Polyclonal Antibody, HRP, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Anti-Plasminogen is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Plasminogen [Human Plasma]
• Cross reactivity against Plasminogen from other sources is unknown
• Plasminogen is a plasma protein synthesized mostly in the liver
• It consists of a single polypeptide chain and has a molecular weight of 88.5 kDa
• The plasma concentration is usually in the range of 70 - 200 mg/L
• Plasminogen is activated by tissue-plasminogen activator, urokinase and streptokinase, to form plasmin
• Activation results from the cleavage and release of the preactivation peptide
• Plasmin consists of a heavy (A-) chain which contains 5 kringles and a light (B-) chain with the active site
• Plasmin is a trypsin-like protease whose natural substrate is fibrin, with binding sites for fibrin residing on the kringles
• Plasmin is essential for fibrinolysis and decreased fibrinolytic potential due to congenital defects in plasmin can lead to recurring thrombosis, thrombophlebitis and pulmonary embolism.