Lactate Dehydrogenase Polyclonal Antibody, HRP, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Anti-Lactate Dehydrogenase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]
• Cross reactivity against Lactate Dehydrogenase from other tissues and species may occur but have not been specifically determined
• Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+
• In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form)
• The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities
• There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure
• The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a)
• LDHB is also known to be upregulated in many cancers
• Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).