50-199-0524
DYKDDDDK Monoclonal Antibody (29E4.G7), Rockland™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-199-0524-Each-of-1 | In Stock | ₹ 14,374.08 |
50-199-0524 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 14,374.08
GST (18%): ₹ 2,587.334
Total Price: ₹ 16,961.414
Antigen
DYKDDDDK
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species
Mouse
Quantity
25 μL
Primary or Secondary
Primary
Product Type
Antibody
Isotype
IgG2a κ
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone
29E4.G7
Conjugate
Unconjugated
Gene Alias
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status
RUO
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Form
Liquid
Target Molecule
Tag
Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- The purified antibody is directed against the FLAGTM motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins
- This monoclonal anti-FLAGTM tag antibody detects over-expressed proteins containing the FLAGTM epitope tag
- In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins
- DYKDDDDK Tag (FLAG™ tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology
- The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended
- A FLAG tag can be used in many different assays that require recognition by an antibody
- If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence
- Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis
- Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein
- The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function
- The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins
- FLAG is a trademark of Sigma-Aldrich Biotechnology LP
Compare Similar Items
Show Difference
Antigen: DYKDDDDK
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 25 μL
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG2a κ
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: 29E4.G7
Conjugate: Unconjugated
Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: Tag
Antigen:
DYKDDDDK
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
25 μL
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG2a κ
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
29E4.G7
Conjugate:
Unconjugated
Gene Alias:
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen:
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
Tag
Antigen: Insulin
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: Dot Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: 2D11.H5
Conjugate: Unconjugated
Gene Alias: AA986540; BOS_25069; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; Insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulin
Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: __
Antigen:
Insulin
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
Dot Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
2D11.H5
Conjugate:
Unconjugated
Gene Alias:
AA986540; BOS_25069; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; Insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulin
Immunogen:
This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
__
Antigen: Keratin
Classification: Monoclonal
Concentration: 1.3 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1
Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Clone: C11
Conjugate: Unconjugated
Gene Alias: 2310016L08Rik; 60-kDa keratin; BB144589; CK6A; CK-6A; CK6B; CK-6B; CK6C; CK-6C; CK6D; CK-6D; CK-6E; cytokeratin 6A; cytokeratin 6B; cytokeratin 6C; cytokeratin 6D; Cytokeratin-6A; Cytokeratin-6B; Cytokeratin-6C; Cytokeratin-6D; Cytokeratin-6E; Hom s 5; K6A; K6B; K6C; K6D; K6E; kamp-keratin derived antimicrobial peptide; KDAMP; Ker2; keratin 6A; keratin 6A, , type II; keratin 6A, type II; keratin 6B; keratin 6B, type II; keratin 6C; keratin 6C, type II; keratin 6E; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6b; keratin complex 2, basic, gene 6c; keratin complex 2, gene 6a; Keratin K6h; keratin, epidermal type II, K6A; keratin, epidermal, type II, K6B; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 6B; Keratin, type II cytoskeletal 6C; Keratin-6A; Keratin-6-alpha; Keratin-6B; keratin-6-beta; Keratin-6C; keratin-like 1 (a type II keratin sequence); Krt2-6; Krt2-6a; Krt2-6b; Krt2-6c; Krt6; KRT6A; KRT6B; KRT6C; KRT6D; KRT6E; KRTL1; mK6[a]; mK6[b]; MK6a; mK6-alpha; mK6-beta; PC2; PC3; PC4; PPKNEFD; Type-II keratin Kb10; Type-II keratin Kb12; Type-II keratin Kb6
Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells.
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: __
Antigen:
Keratin
Classification:
Monoclonal
Concentration:
1.3 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1
Applications:
ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Clone:
C11
Conjugate:
Unconjugated
Gene Alias:
2310016L08Rik; 60-kDa keratin; BB144589; CK6A; CK-6A; CK6B; CK-6B; CK6C; CK-6C; CK6D; CK-6D; CK-6E; cytokeratin 6A; cytokeratin 6B; cytokeratin 6C; cytokeratin 6D; Cytokeratin-6A; Cytokeratin-6B; Cytokeratin-6C; Cytokeratin-6D; Cytokeratin-6E; Hom s 5; K6A; K6B; K6C; K6D; K6E; kamp-keratin derived antimicrobial peptide; KDAMP; Ker2; keratin 6A; keratin 6A, , type II; keratin 6A, type II; keratin 6B; keratin 6B, type II; keratin 6C; keratin 6C, type II; keratin 6E; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6b; keratin complex 2, basic, gene 6c; keratin complex 2, gene 6a; Keratin K6h; keratin, epidermal type II, K6A; keratin, epidermal, type II, K6B; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 6B; Keratin, type II cytoskeletal 6C; Keratin-6A; Keratin-6-alpha; Keratin-6B; keratin-6-beta; Keratin-6C; keratin-like 1 (a type II keratin sequence); Krt2-6; Krt2-6a; Krt2-6b; Krt2-6c; Krt6; KRT6A; KRT6B; KRT6C; KRT6D; KRT6E; KRTL1; mK6[a]; mK6[b]; MK6a; mK6-alpha; mK6-beta; PC2; PC3; PC4; PPKNEFD; Type-II keratin Kb10; Type-II keratin Kb12; Type-II keratin Kb6
Immunogen:
This protein A purified monoclonal antibody was produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells.
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
__
Antigen: Phospho-SMC1 (Ser957)
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Clone: 5D11.G5
Conjugate: Unconjugated
Gene Alias: 14S cohesin SMC1 subunit; 5830426I24Rik; BOS_26006; BOS_6225; CDLS2; Chromosome segregation protein SmcB; DKFZp686L19178; DXhXs423e; DXS423E; KIAA0178; MGC138332; mitosis-specific chromosome segregation protein like protein beta; mKIAA0178; RP6-29D12.1; SB1.8; segregation of mitotic chromosomes 1; segregation of mitotic chromosomes b; segregation of mitotic chromosomes-like 1; SM1A; SMC; SMC (segregation of mitotic chromosomes 1)-like 1; SMC protein; SMC protein 1A; SMC protein 1B; SMC1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 protein cohesin subunit; SMC1 structural maintenance of chromosomes 1-like 1; SMC1 structural maintenance of chromosomes 1-like 2; Smc1a; SMC-1A; smc1a.L; smc1aa; SMC1alpha; SMC-1-alpha; SMC1alpha protein; Smc1b; SMC-1B; SMC1BETA; SMC-1-beta; SMC1L1; SMC1L2; Smcb; SMC-like 1; SMC-protein; structural maintenace of chromosomes 1B; structural maintenance of chromosomes 1; structural maintenance of chromosomes 1 like 1; structural maintenance of chromosomes 1A; structural maintenance of chromosomes 1A a; structural maintenance of chromosomes 1A L homeolog; structural maintenance of chromosomes 1B; Structural maintenance of chromosomes protein 1A; Structural maintenance of chromosomes protein 1B; XELAEV_18000030mg; xsmc1
Immunogen: This antibody was produced from a synthetic peptide corresponding to aa 951-962 of human SMC1 by injection into a balb/c mouse.
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: __
Antigen:
Phospho-SMC1 (Ser957)
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Clone:
5D11.G5
Conjugate:
Unconjugated
Gene Alias:
14S cohesin SMC1 subunit; 5830426I24Rik; BOS_26006; BOS_6225; CDLS2; Chromosome segregation protein SmcB; DKFZp686L19178; DXhXs423e; DXS423E; KIAA0178; MGC138332; mitosis-specific chromosome segregation protein like protein beta; mKIAA0178; RP6-29D12.1; SB1.8; segregation of mitotic chromosomes 1; segregation of mitotic chromosomes b; segregation of mitotic chromosomes-like 1; SM1A; SMC; SMC (segregation of mitotic chromosomes 1)-like 1; SMC protein; SMC protein 1A; SMC protein 1B; SMC1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 protein cohesin subunit; SMC1 structural maintenance of chromosomes 1-like 1; SMC1 structural maintenance of chromosomes 1-like 2; Smc1a; SMC-1A; smc1a.L; smc1aa; SMC1alpha; SMC-1-alpha; SMC1alpha protein; Smc1b; SMC-1B; SMC1BETA; SMC-1-beta; SMC1L1; SMC1L2; Smcb; SMC-like 1; SMC-protein; structural maintenace of chromosomes 1B; structural maintenance of chromosomes 1; structural maintenance of chromosomes 1 like 1; structural maintenance of chromosomes 1A; structural maintenance of chromosomes 1A a; structural maintenance of chromosomes 1A L homeolog; structural maintenance of chromosomes 1B; Structural maintenance of chromosomes protein 1A; Structural maintenance of chromosomes protein 1B; XELAEV_18000030mg; xsmc1
Immunogen:
This antibody was produced from a synthetic peptide corresponding to aa 951-962 of human SMC1 by injection into a balb/c mouse.
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
__