TIMP4 Monoclonal Antibody (9:4-7), Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• TIMP4 antibody is specific for human TIMP4 protein
• This antibody reacts with human TIMP4 in tissue sections of formalin-fixed, paraffin-embedded In ELISAs and Native-PAGE, this antibody does not cross-react with recombinant human TIMP-1, -2 or -3
• A BLAST analysis was used to suggest cross-reactivity with TIMP4 from human and mouse sources based on the immunizing sequence
• Cross-reactivity with TIMP-4 from other sources has not been determined
• Tissue inhibitor of metalloproteinase 4 (TIMP4) is encoded by the gene TIMP4 located on chromosome 3
• It belongs to the family of TIMP genes and is known to inhibit the activity of matrix metalloproteinases
• Matrix metalloproteinases (MMP) are a group of proteinases that degrade the components of extracellular matrix
• TIMP4 irreversibly binds to MMPs and inactivates them with the help of zinc as a cofactor
• The netrin domain of TIMP4 is involved in platelet recruitment and aggregation
• Other biological functions include response to cytokine and peptide hormone stimuli and involvement with the ovulation cycle and CNS development.