50-199-0735
GFP Mouse anti-Tag, DyLight™ 549, Clone: 9F9.F9, Rockland™
Manufacturer: Rockland Immunochemicals
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-199-0735-Each-of-1 | In Stock | ₹ 48,865.03 |
50-199-0735 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 48,865.03
GST (18%): ₹ 8,795.705
Total Price: ₹ 57,660.735
Antigen
GFP
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Product Type
Antibody
Isotype
IgG1 κ
Applications
ELISA, Immunofluorescence, Western Blot
Clone
9F9.F9
Conjugate
DyLight 549
Gene Alias
GFP; GFP tag; GFP2; Turbo eGFP
Immunogen
BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.
Regulatory Status
RUO
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Lyophilized
Target Molecule
Tag
Related Products
Description
- Store vial at 4°C prior to restoration
- For extended storage aliquot contents and freeze at -20°C or below
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum
- Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by Western blot
- No reaction is seen against RFP
- The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal
- GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms
- As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted
- GFP technology has revealed considerable new insights in the physiological activities of living cells
- GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment
- The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors
- GFP fluorescence is stable, species independent and is suitable for a variety of applications
- GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization
- Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
Compare Similar Items
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Antigen: GFP
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: ELISA, Immunofluorescence, Western Blot
Clone: 9F9.F9
Conjugate: DyLight 549
Gene Alias: GFP; GFP tag; GFP2; Turbo eGFP
Immunogen: BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: Tag
Antigen:
GFP
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
ELISA, Immunofluorescence, Western Blot
Clone:
9F9.F9
Conjugate:
DyLight 549
Gene Alias:
GFP; GFP tag; GFP2; Turbo eGFP
Immunogen:
BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
Tag
Antigen: Phospho-AKT (Thr308)
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Clone: 18F3.H11
Conjugate: DyLight 549
Gene Alias: Akt; Akt kinase; AKT serine/threonine kinase 1; AKT/PKB; AKT1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; AKT2; AKT3; CAKT; C-AKT; CG4006; CG4006 gene product from transcript CG4006-RE; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; DAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAkt1; DAKT1/PKB; Dmel\CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; kinase Akt1; l(3)04226; l(3)89Bq; MGC99656; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; p-Akt; Phospho-AKT; Phospho-PKB; PKB; PKB Akt; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; Rac; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein kinases; related to the A and C kinases; serine/threonine protein kinase; serine-threonine protein kinase; STK-2; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: __
Antigen:
Phospho-AKT (Thr308)
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Clone:
18F3.H11
Conjugate:
DyLight 549
Gene Alias:
Akt; Akt kinase; AKT serine/threonine kinase 1; AKT/PKB; AKT1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; AKT2; AKT3; CAKT; C-AKT; CG4006; CG4006 gene product from transcript CG4006-RE; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; DAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAkt1; DAKT1/PKB; Dmel\CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; kinase Akt1; l(3)04226; l(3)89Bq; MGC99656; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; p-Akt; Phospho-AKT; Phospho-PKB; PKB; PKB Akt; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; Rac; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein kinases; related to the A and C kinases; serine/threonine protein kinase; serine-threonine protein kinase; STK-2; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
Immunogen:
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
__
Antigen: 6X His Tag
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: ELISA, Immunofluorescence, Western Blot
Clone: 33D10.D2.G8
Conjugate: DyLight 549
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Immunogen: This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: Tag
Antigen:
6X His Tag
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
ELISA, Immunofluorescence, Western Blot
Clone:
33D10.D2.G8
Conjugate:
DyLight 549
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Immunogen:
This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
Tag
Antigen: GST
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: ELISA, Immunofluorescence, Western Blot
Clone: 3D4
Conjugate: DyLight 649
Gene Alias: Glutathione-S-Transferase; GST protein; GST Tag
Immunogen: BALB/c mice were immunized with GST from full length Schistosoma japonicum protein. A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: Tag
Antigen:
GST
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
ELISA, Immunofluorescence, Western Blot
Clone:
3D4
Conjugate:
DyLight 649
Gene Alias:
Glutathione-S-Transferase; GST protein; GST Tag
Immunogen:
BALB/c mice were immunized with GST from full length Schistosoma japonicum protein. A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
Tag