50-199-0749
DYKDDDDK Mouse anti-Tag, DyLight™ 680, Clone: 29E4.G7, Rockland™
Manufacturer: Rockland Immunochemicals
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-199-0749-Each-of-1 | In Stock | ₹ 45,836.20 |
50-199-0749 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,836.20
GST (18%): ₹ 8,250.516
Total Price: ₹ 54,086.716
Antigen
DYKDDDDK
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Product Type
Antibody
Isotype
IgG2a κ
Applications
ELISA, Flow Cytometry, Immunofluorescence
Clone
29E4.G7
Conjugate
DyLight 680
Gene Alias
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status
RUO
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Lyophilized
Target Molecule
Tag
Description
- Store vial at 4°C prior to restoration
- For extended storage aliquot contents and freeze at -20°C or below
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- This antibody is directed against the FLAGTM epitope tag and is useful in determining its presence in over expressed proteins in various assays
- The antibody recognizes the FLAGTM epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells
- DYKDDDDK Tag (FLAG™ tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology
- The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended
- A FLAG tag can be used in many different assays that require recognition by an antibody
- If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence
- Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis
- Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein
- The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function
- The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins
- FLAG is a trademark of Sigma-Aldrich Biotechnology LP
Compare Similar Items
Show Difference
Antigen: DYKDDDDK
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG2a κ
Applications: ELISA, Flow Cytometry, Immunofluorescence
Clone: 29E4.G7
Conjugate: DyLight 680
Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: Tag
Antigen:
DYKDDDDK
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG2a κ
Applications:
ELISA, Flow Cytometry, Immunofluorescence
Clone:
29E4.G7
Conjugate:
DyLight 680
Gene Alias:
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen:
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
Tag
Antigen: ERK2 Internal
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG
Applications: ELISA, Western Blot
Clone: 3H9.A1.A8
Conjugate: DyLight 680
Gene Alias: 9030612K14Rik; AA407128; AU018647; C78273; ERK; Erk2; ERK-2; ERT1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase-2; extracellular-signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; Mapk; MAPK 1; MAPK 2; MAPK1; MAPK2; mitogen activated protein kinase 1; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p38; p40; p41; p41mapk; p42mapk; p42-MAPK; PRKM1; PRKM2; protein tyrosine kinase ERK2
Immunogen: Anti-ERK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: __
Antigen:
ERK2 Internal
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG
Applications:
ELISA, Western Blot
Clone:
3H9.A1.A8
Conjugate:
DyLight 680
Gene Alias:
9030612K14Rik; AA407128; AU018647; C78273; ERK; Erk2; ERK-2; ERT1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase-2; extracellular-signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; Mapk; MAPK 1; MAPK 2; MAPK1; MAPK2; mitogen activated protein kinase 1; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p38; p40; p41; p41mapk; p42mapk; p42-MAPK; PRKM1; PRKM2; protein tyrosine kinase ERK2
Immunogen:
Anti-ERK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
__
Antigen: GFP
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: ELISA, Immunofluorescence, Western Blot
Clone: 9F9.F9
Conjugate: DyLight 800
Gene Alias: GFP; GFP tag; GFP2; Turbo eGFP
Immunogen: BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: Tag
Antigen:
GFP
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
ELISA, Immunofluorescence, Western Blot
Clone:
9F9.F9
Conjugate:
DyLight 800
Gene Alias:
GFP; GFP tag; GFP2; Turbo eGFP
Immunogen:
BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
Tag
Antigen: 6X His Tag
Classification: Monoclonal
Concentration: __
Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: ELISA, Immunofluorescence, Western Blot
Clone: 33D10.D2.G8
Conjugate: DyLight 405
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Immunogen: This 6X HIS antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: Tag
Antigen:
6X His Tag
Classification:
Monoclonal
Concentration:
__
Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
ELISA, Immunofluorescence, Western Blot
Clone:
33D10.D2.G8
Conjugate:
DyLight 405
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Immunogen:
This 6X HIS antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
Tag