50-199-0777
6X His Tag Mouse anti-Tag, Atto 647N, Clone: 33D10.D2.G8, Rockland™
Manufacturer: Rockland Immunochemicals
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-199-0777-Each-of-1 | In Stock | ₹ 48,865.03 |
50-199-0777 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 48,865.03
GST (18%): ₹ 8,795.705
Total Price: ₹ 57,660.735
Antigen
6X His Tag
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species
Mouse
Quantity
50 μg
Primary or Secondary
Primary
Product Type
Antibody
Isotype
IgG1 κ
Applications
ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Clone
33D10.D2.G8
Conjugate
Atto 647N
Gene Alias
6His tag; 6X His; His Tag; Histidine Tag
Immunogen
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status
RUO
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Lyophilized
Target Molecule
Tag
Description
- Store vial at 4°C prior to restoration
- For extended storage aliquot contents and freeze at -20°C or below
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- 6X HIS Epitope Tag ATTO 647N conjugated antibody is directed against the 6X His motif and is useful in determining its presence in various assays
- This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag
- To date, this antibody has reacted with all His tagged proteins so far tested
- In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins
- The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells
- The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH)
- The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography
- Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering
- By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available
- His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins
- For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent
- His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Compare Similar Items
Show Difference
Antigen: 6X His Tag
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 50 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Clone: 33D10.D2.G8
Conjugate: Atto 647N
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: Tag
Antigen:
6X His Tag
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
50 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Clone:
33D10.D2.G8
Conjugate:
Atto 647N
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Immunogen:
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
Tag
Antigen: Ovalbumin
Classification: Polyclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Rabbit
Quantity: 25 μL
Primary or Secondary: Primary
Product Type: Antibody
Isotype: __
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: __
Conjugate: Unconjugated
Gene Alias: Allergen Gal d II; clade (ovalbumin); Egg albumin; Gal d 2; member 14; OVAL; Ovalbumin; ovalbumin (SERPINB14); Plakalbumin; serpin peptidase inhibitor; SERPINB14
Immunogen: Ovalbumin [Hen Egg White]
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: __
Antigen:
Ovalbumin
Classification:
Polyclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Rabbit
Quantity:
25 μL
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
__
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
__
Conjugate:
Unconjugated
Gene Alias:
Allergen Gal d II; clade (ovalbumin); Egg albumin; Gal d 2; member 14; OVAL; Ovalbumin; ovalbumin (SERPINB14); Plakalbumin; serpin peptidase inhibitor; SERPINB14
Immunogen:
Ovalbumin [Hen Egg White]
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
__
Antigen: alpha Amylase
Classification: Polyclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Rabbit
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: __
Applications: ELISA, Immunoprecipitation, Western Blot
Clone: __
Conjugate: Unconjugated
Gene Alias: 1,4-alpha-D-glucan glucanohydrolase; 1,4-alpha-D-glucan glucanohydrolase 2B; a-amylase; alpha-amylase; Alpha-amylase 2B; Amy2; Amy2-1; Amy2-2; Amy2a; Amy2a1; Amy2a3; AMY2B; AMY3; amylase 2, pancreatic; amylase 2-2, pancreatic; amylase 2a1; amylase 2a3; amylase 2a3, pancreatic; amylase 2b; amylase 2b, pancreatic; amylase, alpha 2A (pancreatic); amylase, alpha 2B (pancreatic); Amy-X; Carcinoid alpha-amylase; glycogenase; HXA; mAmy2-1; mAmy2-4; mAmy2-6; OTTMUSG00000022430; OTTMUSG00000022432; OTTMUSG00000022462; PA; Pancreatic alpha-amylase; pancreatic amylase; pancreatic amylase alpha 2A
Immunogen: a-Amylase [Bacillus amyloliquefaciens]
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: __
Antigen:
alpha Amylase
Classification:
Polyclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Rabbit
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
__
Applications:
ELISA, Immunoprecipitation, Western Blot
Clone:
__
Conjugate:
Unconjugated
Gene Alias:
1,4-alpha-D-glucan glucanohydrolase; 1,4-alpha-D-glucan glucanohydrolase 2B; a-amylase; alpha-amylase; Alpha-amylase 2B; Amy2; Amy2-1; Amy2-2; Amy2a; Amy2a1; Amy2a3; AMY2B; AMY3; amylase 2, pancreatic; amylase 2-2, pancreatic; amylase 2a1; amylase 2a3; amylase 2a3, pancreatic; amylase 2b; amylase 2b, pancreatic; amylase, alpha 2A (pancreatic); amylase, alpha 2B (pancreatic); Amy-X; Carcinoid alpha-amylase; glycogenase; HXA; mAmy2-1; mAmy2-4; mAmy2-6; OTTMUSG00000022430; OTTMUSG00000022432; OTTMUSG00000022462; PA; Pancreatic alpha-amylase; pancreatic amylase; pancreatic amylase alpha 2A
Immunogen:
a-Amylase [Bacillus amyloliquefaciens]
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
__
Antigen: alpha Amylase
Classification: Polyclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Rabbit
Quantity: 25 μL
Primary or Secondary: Primary
Product Type: Antibody
Isotype: __
Applications: ELISA, Immunoprecipitation, Western Blot
Clone: __
Conjugate: Unconjugated
Gene Alias: 1,4-alpha-D-glucan glucanohydrolase; 1,4-alpha-D-glucan glucanohydrolase 2B; a-amylase; alpha-amylase; Alpha-amylase 2B; Amy2; Amy2-1; Amy2-2; Amy2a; Amy2a1; Amy2a3; AMY2B; AMY3; amylase 2, pancreatic; amylase 2-2, pancreatic; amylase 2a1; amylase 2a3; amylase 2a3, pancreatic; amylase 2b; amylase 2b, pancreatic; amylase, alpha 2A (pancreatic); amylase, alpha 2B (pancreatic); Amy-X; Carcinoid alpha-amylase; glycogenase; HXA; mAmy2-1; mAmy2-4; mAmy2-6; OTTMUSG00000022430; OTTMUSG00000022432; OTTMUSG00000022462; PA; Pancreatic alpha-amylase; pancreatic amylase; pancreatic amylase alpha 2A
Immunogen: a-Amylase [Bacillus amyloliquefaciens]
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: __
Antigen:
alpha Amylase
Classification:
Polyclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Rabbit
Quantity:
25 μL
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
__
Applications:
ELISA, Immunoprecipitation, Western Blot
Clone:
__
Conjugate:
Unconjugated
Gene Alias:
1,4-alpha-D-glucan glucanohydrolase; 1,4-alpha-D-glucan glucanohydrolase 2B; a-amylase; alpha-amylase; Alpha-amylase 2B; Amy2; Amy2-1; Amy2-2; Amy2a; Amy2a1; Amy2a3; AMY2B; AMY3; amylase 2, pancreatic; amylase 2-2, pancreatic; amylase 2a1; amylase 2a3; amylase 2a3, pancreatic; amylase 2b; amylase 2b, pancreatic; amylase, alpha 2A (pancreatic); amylase, alpha 2B (pancreatic); Amy-X; Carcinoid alpha-amylase; glycogenase; HXA; mAmy2-1; mAmy2-4; mAmy2-6; OTTMUSG00000022430; OTTMUSG00000022432; OTTMUSG00000022462; PA; Pancreatic alpha-amylase; pancreatic amylase; pancreatic amylase alpha 2A
Immunogen:
a-Amylase [Bacillus amyloliquefaciens]
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
__