p35 Polyclonal Antibody, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This antibody is specific for Borrelia burgdorferi p35 protein
• A BLAST analysis was used to suggest cross-reactivity with p35 from B
• burgdorferi, garinii, and afzelii sources based on 100% homology with the immunizing sequence
• Cross-reactivity with p35 from other sources has not been determined
• The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system
• The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form
• The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions
• P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location
• The p25 form accumulates in the brain neurons of patients with Alzheimer's disease
• This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.