Myeloperoxidase Polyclonal Antibody, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Myeloperoxidase [Human Leukocytes]
• Cross reactivity against Myeloperoxidase from other tissues and species may occur but have not been specifically determined
• Myeloperoxidase (MPO) is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation
• Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains with a molecular weight range of 59 - 64 kDa, and two unglycosylated beta chains (MW ∼14 kDa) with total MW ∼150 kDa and a theoretical pI of 9.2
• Traditionally, myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities such as periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome)
• Low to moderate anti-myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis
• Recently, it was shown that myeloperoxidase participates in the initiation and progression of cardiovascular disease
• Myeloperoxidase possesses potent proinflammatory properties and may contribute directly to tissue injury
• Now Myeloperoxidase is under consideration as one of the most promising cardiac markers
• Myeloperoxidase is part of the host defense system of polymorphonuclear leukocytes and myeloperoxidase is responsible for microbicidal activity against a wide range of organisms
• In the stimulated PMN (polymorphonuclear leukocytes), MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.