Alkaline Phosphatase Polyclonal Antibody, Biotin, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]
• Cross reactivities against Alkaline Phosphatase from other sources may occur but have not been specifically determined
• Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments
• The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment
• ALP plays a role in liver metabolism and development within the skeleton
• In bacteria, ALP acts as a source of inorganic phosphate
• In yeast, ALP can be a useful protein for studying membrane protein sorting.