IL-6Rabbit anti-Human, Polyclonal, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• IL-6 antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie
• IL-1 stimulated IL-6 production from fibroblasts)
• In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule
• The antiserum is also useful for neutralization of human of IL-6 activity in bioassays
• For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested
• A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended
• In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6
• However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity
• This product has minimal reactivity with mouse IL-6
• Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells
• IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response
• IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells
• IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes
• In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes
• IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors
• IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma
• IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas
• Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha
• The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.