AP2AGoat anti-Canine, Chicken, Chimpanzee, Human, Mouse, Non-human primate, Rat, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This affinity purified antibody is directed against human AP2A
• A BLAST analysis was used to suggest reactivity with AP2A1 and AP2A2 protein from human, chimpanzee, orangutan, mouse, rat, chicken and dog based on 100% homology for the immunogen sequence
• Cross reactivity with AP2A proteins from other sources is not known
• Component of the adaptor protein complex 2 (AP-2)
• Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways
• Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation
• AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome
• The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components
• Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation
• AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis
• AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface
• AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules
• AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway
• The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane
• The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins
• The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.